• Tue. Feb 27th, 2024

Exploration of its anticancer, antioxidant, antimicrobial and larvicidal activity

Exploration of its anticancer, antioxidant, antimicrobial and larvicidal activity

In a recently published study Scientific reports In the journal, the researchers explored the use of endophytic fungi to produce selenium nanoparticles.

They reported a successful green synthesis of selenium nanoparticles Penicillium verhagenii Isolated from healthy garlic roots.

Research: Exploring the antimicrobial, antioxidant, anticancer, biocompatibility and larvicidal activities of selenium nanoparticles produced by the endophytic fungal strain Penicillium verhagenii. Image credit: KaterynaKon/Shutterstock.com


Although selenium is one of the essential micronutrients for human health and microbial growth, inorganic and organic selenium compounds have a very narrow range of safe intake, and excessive intake can cause toxicity.

Nanoparticles generally have different physical and chemical properties than their bulk counterparts and are relatively more compatible with organs and tissues. Therefore, selenium nanoparticles are believed to be safer and less toxic to humans than inorganic or organic selenium.

Fungal and plant-related biological pathways have been extensively explored to determine environmentally safe and sustainable nanoparticle production methods.

Recent research has reported that endophytic fungi efficiently produce active metabolites required for sustained production of nanoparticles and produce these metabolites in much higher amounts than non-endophytic fungi.

Furthermore, endophytic fungi not only produce various secondary metabolites such as alkaloids, flavonoids, cyclopeptides and saponins within host plants, but they also share antimicrobial, anti-cancer, anti-inflammatory and other biological properties with host plants.

About the study

The present study used endophytic fungi isolated from healthy garlic roots to explore their nanoparticle biosynthesis potential. Garlic or not Allium sativum An integral part of traditional medicine, the ancient Egyptians used it to treat rhinitis, snake bites, and heart ailments, and the ancient Greeks used garlic for intestinal and respiratory problems. Garlic was used for wounds and ulcers during World War II.

Garlic has various medicinal applications due to its antimicrobial, antifungal, antihypertensive, anticancer, antipyretic, antioxidant and anticonvulsant properties.

AR.1 and AR.4 were among the four endophytic fungal species isolated from garlic roots. Penicillium species, Alternaria alternataAnd Aspergillus niger. Each endophytic fungal strain was separately inoculated and analyzed to determine its effectiveness as a biocatalyst in producing selenium nanoparticles.

Ultraviolet-Visible (UV-Vis) spectroscopy was used to monitor the color change to detect the strain with maximum surface plasmon resonance to identify the strongest strain.

Internal transcribed spacer (ITS) genes of the most virulent strains of endophytic fungi, Penicillium species AR.1 was amplified and sequenced for molecular identification.

Sequences published in the GenBank database were compared with those generated in the present study, and phylogenetic trees were constructed using bioinformatic tools.

In addition, the functional groups in the fungal biomass were examined using Fourier-transformed infrared analysis to determine their effectiveness in reducing and stabilizing the synthesized selenium nanoparticles.

The resulting selenium nanoparticles were further investigated using X-ray diffraction to determine their crystalline structure. Transmission electron microscopy was used to understand the morphological characteristics such as shape, size and aggregation of selenium nanoparticles synthesized through fungal mediation.

The researchers used dynamic light scattering to determine the size of these selenium nanoparticles in the colloidal solution. In contrast, Zeta potential, which determines the electrical charge on the nanoparticle surface, was used to evaluate the stability of the selenium nanoparticle.

The result

UV-Vis spectroscopy and molecular identification results were reported for Penicillium species AR.1. Penicillium verhageniiOf the four strains isolated from garlic roots, it was the most potent.

The resulting selenium nanoparticles were spherical, crystalline, well-ordered between 25 nm and 75 nm, and highly stable.

Selenium nanoparticles are produced P. verhageni-Antimicrobial activity against various pathogens including modulation was also demonstrated Candida albicans and C. tropicalis, C. glabrata, C. parapsilosis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilisAnd Escherichia coli 12.5 µg to 100 µg per milliliter with a low resistance concentration of 100 µg.

Fungal-modulated selenium nanoparticles exhibit antioxidant and anticancer properties while being biocompatible with multiple cell lines. Selenium nanoparticles are effective in many ways Aedes albopictus Larval instars.


Overall, the findings reported that P. Verhageniwas the most potent selenium nanoparticle producer, identified based on ITS gene sequences.

The P. VerhageniThe modulated selenium nanoparticles were spherical and crystalline with sizes between 25 and 75 nm and exhibited antimicrobial, antioxidant, larvicidal activity and cytotoxicity against cancer cells. In vitro. The results confirmed the ability of endophytic fungi to produce active selenium nanoparticles.

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